RNA-sequencing transcriptomic analysis of miR-662-overexpressing BC cells was performed to evaluate gene expression changes.
ALDEFLUOR assays were performed to study BC stemness. The effect of miR-662-overexpressing BC cell conditioned medium on osteoclastogenesis was investigated. Animal models were used to investigate the role of miR-662 in BC (bone) metastasis. MiR-662 was overexpressed following MIMIC-transfection or lentivirus transduction. MiRome analysis in serum from BC patients was performed by TaqMan™ low-density array. Breast cancer (BC) metastasis, which often occurs in bone, contributes substantially to mortality. MicroRNAs play a fundamental role in BC metastasis, although microRNA-regulated mechanisms driving metastasis progression remain poorly understood.
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